Mar Biotechnol (NY). 2014 Apr;16(2):181-92. doi: 10.1007/s10126-013-9532-1. Epub 2013 Sep 18.
Isolation of lactic acid bacteria from kuruma shrimp (Marsupenaeus japonicus) intestine and assessment of immunomodulatory role of a selected strain as probiotic.
Maeda M1, Shibata A, Biswas G, Korenaga H, Kono T, Itami T, Sakai M. 1 Kyushu Medical Co., Ltd., 13-4 Ohte-machi, Kokurakita-ku, Kitakyushu, Fukuoka, 803-0814, Japan.
Fifty-one lactic acid bacteria (LAB) strains were isolated and identified based on 16S ribosomal DNA sequence from the intestinal tracts of 142 kuruma shrimps (Marsupenaeus japonicus) collected from Kanmon Strait, Fukuoka and Tachibana Bay, Nagasaki, Japan. Cellular immunomodulatory function of 51 isolated LAB strains was assessed by measuring the level of interferon (IFN)-γ induction in mouse spleen cell culture. The strain Lactococcus lactis D1813 exhibited the highest amount of IFN-γ production and also bactericidal activity and was selected for testing its immunomodulatory role as a probiotic in kuruma shrimp. We also assessed the effect of dietary incorporation of this probiotic on resistance to Vibrio penaeicida infection in the kuruma shrimp. Our results demonstrate that probiotic L. lactis D1813-containing diet-fed (105 cfu g-1) shrimps displayed a significant up-regulation of lysozyme gene expressions in the intestine and hepatopancreas. However, insignificantly higher expression of anti-lipopolysaccharide factor, super oxide dismutase, prophenoloxidase, and toll-like receptor 1 was recorded in the intestine of shrimps fed the probiotic diet. Moreover, significantly increased (P < 0.01) resistance to the bacterial pathogen in term of better post-infection survival (61.7 %) was observed in the shrimps fed with the probiotic-incorporated diet compared with the control diet-fed group (28.3 %). The present study indicates the immunomodulatory role of the LAB L. lactis D1813 on the kuruma shrimp immune system and supports its potential use as an effective probiotic in shrimp aquaculture.
The positive contributions of PowerLac™ supplementation to the production performance, feed utilization and disease resistance of Nile tilapia Oreochromis niloticus (L.)
Muhammad A Suprayudi, Minoru Maeda, Hidayatullah Hidayatullah, Widanarni Widanarni, Mia Setiawati & Julie Ekasari
The objective of this research was to determine the optimum dietary supplementation level of a probiotic (PowerLac™), for improving Nile tilapia Oreochromis niloticus growth performance and feed utilization, as well as enhanced protection against disease. For lab- oratory experimentation, a completely randomized experimental design, consisting of four treatments of a dietary probiotic (PowerLac™, containing Lactobacillus lactis D1813) supplementation at different levels (0.25, 0.5, 1.0 and 2.0 g kg-1), against acontrol (0 g kg-1) was performed for 8 weeks (of culture period). For field experiments, three treat- ments of different dietary probiotic supplementation levels (0.25, 0.5 and 1.0 g kg-1), plus a control, were employed for 22 weeks (of culture period). Under controlled experimental conditions, in the first experiment, significantly higher fish final body weight, growth and lower food conversion ratio (FCR) were achieved by treatments with dietary pro- biotic supplementations of 0.25 and 0.5 g kg-1(P < 0.05). In strong partial support of this, at field experimentation level, a dietary PowerLac™ supple- mentation of 0.5 g kg-1 showed the most pronounced results, as indicated by the higher growth, and protein and lipid retention, as well as lower FCR, and reduced mortality following the Aeromonas hydrophila challenge test (P < 0.05).